Cas9 gRNA engineering for genome editing activation and repression

Nature Methods
Authors
Samira Kiani1,2,11, Alejandro Chavez3–5,11,
Marcelle Tuttle3, Richard N Hall1,2, Raj Chari5,
Dmitry Ter-Ovanesyan3,5, Jason Qian3,5,
Benjamin W Pruitt3, Jacob Beal6, Suhani Vora1,3,5, Joanna Buchthal3, Emma J K Kowal3,
Mohammad R Ebrahimkhani1,7, James J Collins1–3,8–10, Ron Weiss1,2,7 & George Church3,5
11-04-2015
12:00pm
PST
Categories
RNA Modification & Editing
Speaker
Ryan Hromyak
Abstract
We demonstrate that by altering the length of Cas9- associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.