Carol Oxford - ThermoFisher

Tips and Techniques for Optimizing Multiparameter Flow Cytometry Panels
Multiparameter flow cytometry continues to become more complex as new dyes are developed not only for surface immunophenoytping, but for functional assays, intracellular protein expression, and now gene expression. As more number of parameters are included, characterization of instrument performance and understanding of dye interactions becomes critical for detection of dimly expressed markers. Optimization of instrument voltages, and understanding of which detectors should be used to make the most sensitive measurements is critical. Instrument characterization and measurement of spillover spreading can make panel design easier, and optimize sensitivity of flow cytometry experiments.
October 4, 2017
HSW 1057 at noon