Eleonora De Klerk

Chromatin-enriched lncRNAs can act as cell-type specific activators of proximal gene transcription
Werner MS1, Sullivan MA1,2, Shah RN1,2, Nadadur RD3, Grzybowski AT1, Galat V4, Moskowitz IP3, Ruthenburg AJ1,2.
Nat Struct Mol Biol. 2017 Jul;24(7):596-603. doi: 10.1038/nsmb.3424. Epub 2017 Jun 19.
July 1, 2017
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, USA. Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA. Department of Pediatrics and Pathology, The University of Chicago, Chicago, Illinois, USA. Department of Pathology, Stanley Manne Children's Research Institute, Ann and Robert H. Lurie Children's Hospital of Chicago, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
We recently described a new class of long noncoding RNAs (lncRNAs) that are distinguished by especially tight chromatin association and whose presence is strongly correlated to expression of nearby genes. Here, we examine the cis-enhancer mechanism of this class of chromatin-enriched RNA (cheRNA) across multiple human cell lines. cheRNAs are largely cell type specific and provide the most reliable chromatin signature to predict cis-gene transcription in every human cell type examined. Targeted depletion of three cheRNAs decreases expression of their neighboring genes, indicating potential co-activator function, and single-molecule fluorescence in situ hybridization (smFISH) of one cheRNA-distal target gene pair suggests a spatial overlap consistent with a role in chromosome looping. Additionally, the cheRNA HIDALGO stimulates the fetal hemoglobin subunit gamma 1 (HBG1) gene during erythroid differentiation by promoting contacts to a downstream enhancer. Our results suggest that multiple cheRNAs activate proximal lineage-specific gene transcription.
Date: 
August 30, 2017
Where: 
HSW 1057 at noon