Multiplexed quantification of proteins and transcripts in single cells
Nat Biotechnol. 2017 Oct;35(10):936-939. doi: 10.1038/nbt.3973. Epub 2017 Aug 30.
https://www.nature.com/articles/nbt.3973.pdf
John Gagnon
Time
12:00pm
We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type.
RNA Nanotechnology
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